Etude du vivant (biologie et biomedical)

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Non-linear optics for molecular spectroscopy

Principal investigator : Julien Duboisset

Key words : Coherent anti-Stokes Raman scattering, spontaneous Raman, polarization, molecular order, vibrational modes.

We are particularly interested in investigate the molecular vibration for biological imaging and fundamental nonlinear spectroscopy. We develop method based on spontaneous Raman and coherent Raman scattering to directly image the symmetry of molecules in crystals and biological tissues, based on irreducible tensor formalism.

Since 2014, we are able to perform vibrational modes imaging in crystal, and assign their modes symmetry in single image acquisition. We are also able to perform molecular organization imaging in biological tissue and soft matter.




C. Cleff, A. Gascka, P. Ferrand, H. Rigneault , S. Brasselet, J. Duboisset
Direct imaging of molecular symmetry by coherent anti-Stokes Raman scattering


J. Duboisset, P. Berto, P. Gasecka, F..Z. Bioud, P. Ferrand, H. Rigneault , S. Brasselet
Molecular orientational order probed by coherent anti-Stokes Raman scattering (CARS) and stimulated Raman Scattering (SRS) microscopy : a spectral comparative study
J. Phys. Chem. B 119 (7), pp 3242–3249 (2015)


J. Duboisset, H. Rigneault, S. Brasselet
‘Filtering of matter symmetry properties by circularly polarized nonlinear optics
Phys. Rev. A 90, 063827 (2014)
F.-Z. Bioud, P. Gasecka, P. Ferrand, H. Rigneault, J. Duboisset, and S. Brasselet
Structure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman scattering microscopy
Phys. Rev. A 89, 013836 (2014)