{"id":1890,"date":"2024-07-02T11:18:40","date_gmt":"2024-07-02T09:18:40","guid":{"rendered":"https:\/\/www.fresnel.fr\/wp\/?post_type=mosaic&p=1890"},"modified":"2026-02-02T13:13:03","modified_gmt":"2026-02-02T12:13:03","slug":"polarized-microscopy","status":"publish","type":"mosaic","link":"https:\/\/www.fresnel.fr\/wp\/en\/mosaic\/themes\/polarized-microscopy\/","title":{"rendered":"Polarized Microscopy"},"content":{"rendered":"

Polarization Microscopy: from single molecules to tissues<\/span><\/strong>
\nPrincipal investigators<\/b>\u00a0:\u00a0Sophie Brasselet, Miguel Alonso and Luis Aleman-Castaneda<\/p>\n

Keywords<\/b> : polarized fluorescence, polarized super-resolution imaging, polarized nonlinear microscopy<\/p>\n

Polarization is an important property of light that is key for how light interacts with matter (for example, reflection, refraction, scattering, fluorescence, etc.). However, polarization is often ignored, in particular for bio-imaging applications. We develop methodologies for optical microscopy that exploit the polarization of light by controling the incident or detected polarization states. These approaches allow retrieving molecular orientational information in biological media, from complex bio-molecular assemblies down to single molecules. The methodology is also applied for the study of nanostructures, either dielectric or metallic.<\/p>\n

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Excitation fluorescence approaches<\/strong><\/p>\n

Although these techniques are not compatible with single molecule measurements, they are the most commonly used for bio-molecular assemblies. These techniques exploit the fact that fluorescence is maximized when the polarization state of the excitation laser matches the orientation of molecules. For each pixel of an image, we probe the 2D orientation (projection onto the sample plane) and degree of organization of fluorophores attached to proteins of interest. This approach has been successfully applied to studying the organization of cell membrane lipids and actin filaments, and it can be extended to examine orientational changes in membrane proteins, for example. Additionally, probing molecular organization has great potential for visualizing cytoskeletal filament reorganization, such as during morphogenetic events, in real time at the molecular scale. This can help elucidate how these structures contribute to the mechanical properties of cells and tissues, and reveal the roles of associated regulatory proteins. \u00a0A major part of this work is currently carried out in collaboration with Manos Mavrakis (MOSAIC team), who studies cytoskeletal proteins such as actin and septin. Lastly, the freely shareable (open source) data analysis software, PyPOLAR, has been co-developed with the Mathematics Institute of Marseille (I2M).<\/p>\n

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Detection fluorescence approaches<\/strong><\/p>\n

These approaches exploit the distinct fluorescence emission, both in intensity and polarization, that depends on the orientation of each emitter. These approaches can be used with single molecule measurements, meaning that (1) they are compatible with super-resolution imaging (~10 nm by e.g. STORM, PALM, PAINT), and \u00a0that (2) they can offer information on the mean orientation and wobbling (orientational flexiblitiy) of the single emitters. Note that these techniques can offer easily 3D orientation information compared to the excitation approaches. We have shown the possiblility of reconstructing super-resolution orientation images using their localization information, while the orientational flexibility is an important parameter to quantify that we are able to retrieve realistic structural information on the labeled bio-molecules. This approach has been applied to organized filaments such as dsDNA, actin stress fibers, microtubules. Currently developed and exploited techniques are PSF engineering techniques, such as CHIDO and C3POL, and ratiometric techniques either based on polarization splitting, such as 4Polar and 4Polar 3D, or BFP splitting.<\/p>\n

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Polarized non-linear imaging<\/strong><\/p>\n

Due to the nonlinear vectorial coupling between incident light polarizations and molecular bonds \/ molecular induced dipoles directions, tunable incident polarizations lead to a modulation of nonlinear label-free signals (Second Harmonic Generation\u00a0: SHG, Coherent Anti Stokes Raman Scattering\u00a0: CARS, Stimulated Raman Scattering\u00a0: SRS) that can be directly related to the orientational order of biological molecules, without the need of a fluorescent label. We have applied polarized nonlinear microscopy to quantitatively retrieve organizational order in collagen in tissues (by polarized SHG) and lipid structures (by polarized CARS). This methodology is highly sensitive to lipid phases but also to sub-diffraction scale morphological changes in cell membranes. Polarization resolved CARS (pCARS) is now used to image the effect of neurodegenerative diseases on fine myelin structure changes, in mice spinal cord tissues. We are currently developing schemes that allow polarized nonlinear imaging to reach fast imaging rates, either by fast polarization tuning, or via the use of projection on circular polarization combinations. We are also studying the possibilities to develop optical schemes capable to measure the orientation of molecular distributions in 3D, an information which is important in complex tissues. All together these approaches should permit to better quantify molecular order in live and in 3D in tissues related to the study of diseases such as neurodegenerative diseases or cancer.<\/p>\n

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Theoretical optics of polarization<\/strong><\/p>\n

A good theoretical understanding of polarization in the paraxial and nonparaxial regimes is essential for the appropriate estimation and characterization of molecule orientation and wobbling, their effect on the captured images, and the optimization of the imaging systems used to measure them. We study different theoretical formalisms for characterizing the polarization of nonparaxial electromagnetic fields, for which the field oscillations are not constrained to a plane normal to the direction of propagation. We seek for geometric descriptions of these oscillations, valid in different regimes, on the one hand to understand the inherent dimensionality, geometry and topology of these spaces, while on the other to optimize their characterization. We also explore different aspects of nonparaxial polarization of interest beyond fluorescence microscopy, such as the presence of transverse or longitudinal spin, interpretations for geometric phases, or types of electromagnetic field that are analogues of skyrmions.<\/p>\n

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Developped polarized microscopy techniques<\/span><\/strong>
\nFluorescence and nonlinear imaging based on excitation schemes:<\/strong>
\n\u2013 Spinning disk fluorescence microscopy (live polarization imaging)
\n\u2013 Nonlinear scanning polarized microscopy (2-photon fluorescence, SHG, CARS, SRS)<\/p>\n

Super-resolution and single molecule microscopy based on detection schemes :<\/strong>
\n\u2013 2D Polarized STORM microscopy
\n\u2013 3D polarized STORM microscopy (using polarization splitting, BFP splitting or PSF engineering)<\/p>\n

 <\/p>\n

Publications<\/span><\/strong><\/p>\n

2025<\/strong><\/p>\n

Brasselet S, Lew MD. , Single molecule orientation and localization microscopy,<\/em>\u00a0Nat Photonics<\/strong>. 2025 Sep;19:925-937. doi: 10.1038\/s41566-025-01724-y<\/a><\/p>\n

Martins CS, Iv F, Suman SK, Panagiotou TC, Sidor C, Ruso-L\u00f3pez M, Plancke CN, Omi S, Pag\u00e8s R, Gomes M, Llewellyn A, Bandi SR, Ramond L, Arbizzani F, Rimoli CV, Schnorrer F, Robin F, Wilde A, LeGoff L, Pedelacq JD, J\u00e9gou A, Cabantous S, Rincon SA, Chandre C, Brasselet S, Mavrakis M. Genetically encoded reporters of actin filament organization in living cells and tissue<\/em>s. Cell<\/strong>. 2025 May 1;188(9):2540-2559.e27. doi:10.1016\/j.cell.2025.03.003<\/a><\/p>\n

2024<\/strong><\/p>\n

Herrera I, Alem\u00e1n-Casta\u00f1eda LA, Brasselet S, Alonso MA. Stokes-based analysis for the estimation of 3D dipolar emission<\/em>. J Opt Soc Am A Opt Image Sci Vis<\/strong>. 2024 Nov 1;41(11):2134-2148. doi:10.1364\/JOSAA.538706<\/a><\/p>\n

R. Guti\u00e9rrez-Cuevas, L. Alem\u00e1n-Casta\u00f1eda, I. Herrera, S. Brasselet, M. Alonso, Vectorial phase retrieval in super-resolution polarization microscopy. APL Photonics 9, 026106 (2024), doi.org\/10.1063\/5.0179906<\/a>\u00a0<\/p>\n

D Marco, I Herrera, S Brasselet, MA Alonso, Propagation-invariant optical meron lattices (2024). arXiv:2402.08650 (2024). ACS Photonics 11(6) pp 2397-2405 (2024). doi.org\/10.1021\/acsphotonics.4c00292<\/a><\/p>\n

D. Marco, I. Herrera, S. Brasselet, and M. A. Alonso. Periodic skyrmionic textures via conformal cartographic projections. APL Photonics 9, 110803 (2024). https:\/\/doi.org\/10.1063\/5.0230959<\/a><\/p>\n

2023<\/strong><\/p>\n

Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanovi\u0107 V, Omi S, Pedelacq JD, Iv F, Bouillard L, Llewellyn A, Gomes M, Belhabib M, Kuzmi\u0107 M, Verdier-Pinard P, Lee S, Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, Koenderink GH, Wenger J, Cabantous S, Mavrakis M. Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells<\/em>. J Cell Biol<\/strong>. 2023 Mar 6;222(3):e202203016. doi: 10.1083\/jcb.202203016<\/a><\/p>\n

Nuzhdin D, Pendleton EG, Munger EB, Mortensen LJ, Brasselet S. In-depth polarisation resolved SHG microscopy in biological tissues using iterative wavefront optimisation<\/em>. J Microsc.<\/strong> 2023 Jul;291(1):57-72. doi: 10.1111\/jmi.13163<\/a>.<\/p>\n

E. Munger, M. Sison and S. Brasselet. Influence of the excitation polarization on single molecule 3D orientation imaging. Opt. Communications (541), 129480 (2023). doi.org\/10.1016\/j.optcom.2023.129480<\/a><\/p>\n

S. Brasselet, M.A. Alonso, Polarization microscopy: from ensemble structural imaging to single molecule 3D orientation and localization microscopy. Minireview. Optica 10 (11), 1486-1510 (2023) doi.org\/10.1364\/OPTICA.502119<\/a><\/p>\n

2022<\/strong><\/p>\n

Brasselet S. Fluorescence polarization modulation super-resolution imaging provides refined dynamics orientation processes in biological samples<\/em>. Light Sci Appl.<\/strong> 2022 Nov 7;11(1):322. doi: 10.1038\/s41377-022-01018-w<\/a>.<\/p>\n

Brasselet S. Unraveling the geometry of complex protein organizations by polarized fluorescence imaging.<\/em> Biophys J<\/strong>. 2022 Nov 15;121(22):4242-4243. doi: 10.1016\/j.bpj.2022.09.022<\/a>.<\/p>\n

Alem\u00e1n-Casta\u00f1eda LA, Feng SY, Guti\u00e9rrez-Cuevas R, Herrera I, Brown TG, Brasselet S, Alonso MA. Using fluorescent beads to emulate single fluorophores<\/em>. J Opt Soc Am A Opt Image Sci Vis<\/strong>. 2022 Dec 1;39(12):C167-C178. doi: 10.1364\/JOSAA.474837<\/a><\/p>\n

Yang X, Li C, Giorgi M, Siri D, Bugaut X, Chatelet B, Gigmes D, Yemloul M, Hornebecq V, Kermagoret A, Brasselet S, Martinez A, Bardelang D. Energy-Efficient Iodine Uptake by a Molecular Host\u22c5Guest Crystal<\/em>. Angew Chem<\/strong> Int Ed Engl. 2022 Dec 5;61(49):e202214039. doi: 10.1002\/anie.202214039<\/a><\/p>\n

Adamczyk AK, Huijben TAPM, Sison M, Di Luca A, Chiarelli G, Vanni S, Brasselet S, Mortensen KI, Stefani FD, Pilo-Pais M, Acuna GP. DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation<\/em>. ACS Nano.<\/strong> 2022 Oct 25;16(10):16924-16931. doi: 10.1021\/acsnano.2c06936<\/a>.<\/p>\n

Arbizzani F, Mavrakis M, Hoya M, Ribas JC, Brasselet S, Paoletti A, Rincon SA. Septin filament compaction into rings requires the anillin Mid2 and contractile ring constriction<\/em>. Cell Rep.<\/strong> 2022 Apr 19;39(3):110722. doi: 10.1016\/j.celrep.2022<\/a>.<\/p>\n

Rimoli CV, Valades-Cruz CA, Curcio V, Mavrakis M, Brasselet S. 4polar-STORM polarized super-resolution imaging of actin filament organization in cells<\/em>. Nat Commun.<\/strong> 2022 Jan 13;13(1):301. doi: 10.1038\/s41467-022-27966-w<\/a><\/p>\n

2021<\/strong>
\nJ.-Y. Wang, J.C. Mansfield, S. Brasselet, C. Vergari, J.R. Meakin, C.P. Winlove.
\nMicro-Mechanical Damages of Needle Puncture on Bovine Annulus Fibrosus Fibrils Studies using 1 Polarisation-Resolved Second Harmonic Generation(P-SHG) Microscopy.<\/i>
\nJournal of the Mechanical Behavior of Biomedical Materials<\/b>\u00a0(2021)<\/p>\n

Gasecka P, Balla NK, Sison M, Brasselet S. Lipids-Fluorophores Interactions Probed by Combined Nonlinear Polarized Microscopy.<\/em> J Phys Chem B<\/strong>. 2021 Dec 23;125(50):13718-13729. doi: 10.1021\/acs.jpcb.1c07866<\/a>.<\/p>\n

2020<\/strong>
\nV. Curcio, L. A. Aleman-Castaneda, T. G. Brown, S. Brasselet, M.\u00a0A. Alonso,
\nBirefringent Fourier filtering for single molecule Coordinate and Height super-resolution Imaging with Dithering and Orientation (CHIDO).<\/i>
\n\u00a0Nat. Communications<\/b>\u00a011 (1) (2020)
\nDOI\u00a0: 10.1038\/s41467-020-19064-6<\/a><\/p>\n

2019<\/strong>
\nN. Mazumder, N K. Balla, G-Y Zhuo, Y V. Kistenev, R Kumar, F-J Kao, S Brasselet, V V. Nikolaev, N A. Krivova.
\nLabel-free Nonlinear Multimodal Optical Microscopy \u2013 Basics, Development and Applications,<\/i>
\nFrontiers in Physics<\/b>, Review, 2019
\nDOI\u00a0: 10.3389\/fphy.2019.00170 (2019)<\/a><\/p>\n

M.A. Juanes, D. Isnardon, A. Badache, S. Brasselet, M.\u00a0Mavrakis, B. Goode,
\nThe role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover,<\/i>
\nJ. Cell Bio.<\/b>\u00a0(2019)
\nDOI\u00a0: 10.1083\/jcb.201904165<\/a><\/p>\n

J. Rouxel, H. Shen, N. Nguyen, S. Brasselet, T. Toury.
\nEnhanced second harmonic generation of gold nanostars\u00a0: optimizing multipolar radiation to improve nonlinear properties,<\/i>
\nOpt. Express<\/b>\u00a027(4), 5620-5640 (2019)
\nDOI\u00a0: 10.1364\/OE.27.005620<\/a><\/p>\n

C. Rendon-Barraza, F. Timpu, R. Grange, S. Brasselet.
\nCrystalline heterogeneity in single ferroelectric nanocrystals revealed by polarized nonlinear microscopy.<\/i>
\nSci. Reports\u00a0<\/b>9, 1670 (2019)
\nDOI\u00a0: 10.1038\/s41598-018-38229-4<\/a><\/p>\n

J. Mansfield, V. Mandalia, A. Toms, C. P. Winlove, S. Brasselet.
\nCollagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy<\/i>
\nJournal of the Royal Society Interface\u00a0<\/b>16 (150), pp.20180611 (2019)
\nDOI\u00a0: 10.1098\/rsif.2018.0611<\/a><\/p>\n

S. Brasselet
\nPolarized Microscopy in the Life Sciences.<\/i>
\nOptics&Photonics News\u00a0<\/b>(2019)<\/p>\n

https:\/\/www.osa-opn.org\/home\/articles\/volume_30\/april_2019\/features\/polarization-resolved_microscopy_in_the_life_scien\/<\/a><\/p>\n

2018<\/strong>
\nN. Chouaki-Benmansour, K. Ruminski, A.-M.\u00a0Sartre, M.-C. Phelipot, A. Salles, E. Bergot, A. Wu, G. Chicanne, M.\u00a0Fallet, S. Brustlein, C. Billaudeau, A. Formisano, S. Mailfert, B. Payrastre, D. Marguet, S. Brasselet, Y. Hamon, H.T. He
\nPhosphatidylinositol 4,5-bisphosphate regulates the TCR\/CD3 complex membrane dynamics and activation<\/i>,
\nSci. Reports\u00a0<\/b>8, 4966 (2018)
\ndoi: org\/10.1038\/s41598-018-23109-8<\/p>\n

N. K. Balla, M.\u00a0O\u2019Brien, N. McEvoy, G. S. Duesberg, H. Rigneault, S. Brasselet, D. McCloskey.
\nEffects of Excitonic Resonance on Second and Third Order Nonlinear Scattering from Few-Layer MoS2.<\/i>
\nACS Photonics\u00a0<\/b>, 5 (4), pp 1235\u20131240 (2018)
\nDOI\u00a0: 10.1021\/acsphotonics.7b00912<\/p>\n

O. Loison, M.\u00a0Weitkunat, A. Kaya-\u00c7opur, C. Nascimento Alves, T. Matzat, M.\u00a0L. Spletter, S. Luschnig, S. Brasselet, P.-F. Lenne and F. Schnorrer,
\nPolarization resolved microscopy reveals a muscle myosin motor independent mechanism of molecular actin ordering during sarcomere maturation.<\/i>
\nPLoS Biol\u00a0<\/b>16(4)\u00a0: e2004718 (2018)
\nDOI\u00a0: 10.1371\/journal.pbio.2004718<\/p>\n

2017<\/strong>
\nH. A. Shaban, C. A. Valades-Cruz, J. Savatier, S. Brasselet,
\nPolarized super-resolution structural imaging inside amyloid fibrils using Thioflavine T,<\/i>
\nScientific Reports\u00a0<\/b>7\u00a0: 12482 (2017)
\nDOI:10.1038\/s41598-017-12864<\/p>\n

P. Gasecka, A. Jaouen, F.-Z. Bioud, H. Barbosa de Aguiar, J. Duboisset, P. Ferrand, H. Rigneault, N. Balla, F. Debarbieux, S. Brasselet,
\nDegradation of molecular organization of myelin lipids in autoimmune demyelination probed by polarization resolved nonlinear vibrational microscopy,<\/i>
\nBiophys. J.\u00a0<\/b>113 (7), p1520\u20131530 (2017).
\nDOI\u00a0: 10.1016\/j.bpj.2017.07.033<\/p>\n

H.B. De Aguiar, S. Gigan, S. Brasselet,
\nPolarization recovery through scattering media,<\/i>
\nScience Advances\u00a0<\/b>3 (9), e1600743\u00a0; (2017),
\nDOI\u00a0: 10.1126\/sciadv.1600743<\/p>\n

C. Cleff, H. Rigneault, S. Brasselet, J. Duboisset,
\nNonlinear optical susceptibility described with a spherical formalism, applied to coherent anti-Stokes Raman scattering,<\/i>
\nPhys. Rev. A\u00a0<\/b>96, 013851 (2017).
\nDOI\u00a0: 10.1103\/PhysRevA.94.059902<\/p>\n

M.\u00a0Hofer, N.K. Balla, S. Brasselet,
\nHigh speed polarization resolved Coherent Raman Scattering imaging,<\/i>
\nOptica\u00a0<\/b>4 (7), pp. 795-801 (2017).
\nDOI\u00a0: 10.1364\/OPTICA.4.000795.<\/p>\n

Highlighted in Biophotonics.World (https:\/\/www.biophotonics.world\/magazine\/article\/181\/new-imaging-technique-able-to-watch-molecular-dynamics-of-neurodegenerative-diseases<\/a>) and OSA news release (http:\/\/www.osa.org\/en-us\/about_osa\/newsroom\/news_releases\/2017\/new_imaging_technique_fast_enough_to_watch_molecul\/<\/a>)<\/i><\/p>\n

X. Wang, F. Yang, J. Yin, P. Ferrand, S. Brasselet,
\nQuantifying the polarization properties of non-depolarizing optical elements with virtual distorting elements,<\/i>
\nApplied Optics\u00a0<\/b>56 (10) :2589 (2017)<\/p>\n

N.K. Balla, C. Rendon-Barraza, L.M.\u00a0Hoang, P. Karpinski, E. Bermudez, S. Brasselet,
\nPolarized nonlinear nanoscopy of metal nanostructures,<\/i>
\nACS Photonics\u00a0<\/b>4 (2), pp 292\u2013301 (2017).
\nDOI:10.1021\/acsphotonics.6b00635<\/p>\n

2015<\/strong>
\nH. B. de Aguiar, P. Gasecka and S. Brasselet
\nQuantitative analysis of light scattering in polarization-resolved nonlinear microscopy<\/i>
\nOpt. Express<\/b>\u00a023 (7), pp. 8960-8973 (2015)<\/p>\n

J. Duboisset, P. Berto, P. Gasecka, F..Z. Bioud, P. Ferrand, H. Rigneault , S. Brasselet
\nMolecular orientational order probed by coherent anti-Stokes Raman scattering (CARS) and stimulated Raman Scattering (SRS) microscopy\u00a0: a spectral comparative study<\/i>
\nJ. Phys. Chem. B<\/b>\u00a0119 (7), pp 3242\u20133249 (2015)<\/p>\n

V. Wasik, P. R\u00e9fr\u00e9gier, M.\u00a0Roche and S. Brasselet
\nPrecision of polarization-resolved second harmonic generation microscopy limited by photon noise for samples with cylindrical symmetry<\/i>
\nJOSA A<\/b>\u00a032(8) 1437-1445 (2015)<\/p>\n

2014<\/strong>
\nJ. Duboisset, H. Rigneault, S. Brasselet
\nFiltering of matter symmetry properties by circularly polarized nonlinear optics<\/i>
\nPhys. Rev. A<\/b>\u00a090, 063827 (2014)<\/p>\n

M.\u00a0Mavrakis, Y. Azou-Gros, F-C. Tsai, J. Alvarado, A. Bertin, F. Iv, A. Kress, S. Brasselet, G.H. Koenderink and T. Lecuit
\nSeptins promote F-actin ring formation by cross-linking actin filaments into curved bundles<\/i>
\nNature Cell Biology<\/b>\u00a016, 322\u2013334 (2014)<\/p>\n

F.-Z. Bioud, P. Gasecka, P. Ferrand, H. Rigneault, J. Duboisset, and S. Brasselet
\nStructure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman scattering microscopy<\/i>
\nPhys. Rev. A<\/b>\u00a089, 013836 (2014)<\/p>\n

P. Ferrand, P. Gasecka, A. Kress, X. Wang, F.-Z. Bioud, J. Duboisset, S. Brasselet
\nUltimate use of two-photon fluorescence microscopy to map fluorophores orientational behavior<\/i>
\nBiophys. J.<\/b>\u00a0106 2330\u20132339 (2014)<\/p>\n

2013<\/strong>
\nA. Kress, X. Wang, H. Ranchon, J. Savatier, H. Rigneault, P. Ferrand, S. Brasselet
\nMapping the local organization of cell membranes using generalized polarization resolved confocal fluorescence microscopy<\/i>
\nBiophys. J.<\/b>\u00a0105, 127-136 (2013)<\/p>\n

H. Shen, N. Nguyen, D. Gachet, V. Maillard, T. Toury, S. Brasselet
\nNanoscale optical properties of metal nanoparticles probed by Second Harmonic Generation microscopy<\/i>
\nOpt. Express<\/b>\u00a021 (10), pp.12318-12326 (2013)<\/p>\n

X. Wang, A. Kress, S. Brasselet, P. Ferrand
\nHigh frame-rate confocal angular resolved linear dichroism fluorescence microscopy<\/i>
\nRev. Sc. Instr. Rev. Sci. Instrum.<\/b>\u00a084, 053708 (2013)<\/p>\n

J. Duboisset, P. Ferrand, H. Wei, X. Wang, H. Rigneault, S. Brasselet
\nThioflavine-T and Congo Red Reveal the Polymorphism of Insulin Amyloid Fibrils when Probed by Polarization-Resolved Fluorescence Microscopy<\/i>
\nJ. Phys. Chem. B<\/b>, 2013, 117 (3), pp 784\u2013788<\/p>\n

2012<\/strong>
\nS. Brasselet, P. Ferrand, A. Kress, X. Wang, H. Ranchon, A. Gasecka
\nImaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy<\/i>
\nY. M\u00e9ly and G. Duportail (eds.), Fluorescent Methods to Study Biological Membranes, Springer Series Fluorescence
\nSpringer-Verlag Berlin Heidelberg 2012, 13, 311-338 (2013)<\/p>\n

P. Refregier, M.\u00a0Roche, J. Duboisset, S. Brasselet
\nPrecision increase with two orthogonal analyzers in polarization resolved second harmonic generation microscopy<\/i>
\nOpt. Lett.<\/b>\u00a037 (20), pp.4173-4175 (2012)<\/p>\n

D. Ait-Belkacem, M.\u00a0Guilbert, M.\u00a0Roche, J. Duboisset, P. Ferrand, G. Sockalingum, P. Jeannesson, and S. Brasselet
\nMicroscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation<\/i>
\nJ. Biomed. Opt.<\/b>\u00a017, 080506 (2012)<\/p>\n

F. Munhoz, H. Rigneault, S. Brasselet
\nPolarization\u2013resolved four-wave mixing for structural imaging in thick tissues<\/i>
\nJ. Opt. Soc. Am. B<\/b>\u00a029 (6), pp.1541-1550 (2012)<\/p>\n

J. Duboisset, Dora A\u00eft-Belkacem, Muriel Roche, Herv\u00e9 Rigneault, Sophie Brasselet
\nGeneric model of the molecular orientational distribution probed by polarization resolved Second Harmonic Generation<\/i>
\nPhys. Rev. A.<\/b>\u00a085, 043829-38 (2012)<\/p>\n

A. Gasecka, P. Tauc, A. Bentley, S. Brasselet
\nInvestigation of Molecular and Protein Crystals by Three Photon Polarization Resolved Microscopy<\/i>
\nPhys Rev Lett<\/b>\u00a0108, 263901-05 (2012)<\/p>\n

F. Munhoz, S. Brustlein, R. Hostein, P. Berto, S. Brasselet and H. Rigneault
\nPolarization resolved stimulated Raman scattering\u00a0: probing depolarization ratios of liquids<\/i>
\nJournal of Raman Spectroscopy<\/b>\u00a043 (3), 419\u2013424 (2012)<\/p>\n

S. Monneret, S. Brasselet
\nAdvanced microscopy techniques for biological imaging<\/i>
\nInt. J. Nanotechnol.<\/b>, Vol. 9 (3\u20137), 548-566 (2012)<\/p>\n

2011<\/strong>
\nA. Kress, P. Ferrand, H. Rigneault, T. Trombik, H.-T. He, D. Marguet, S. Brasselet
\nProbing orientational behavior of MHC Class I protein and lipid probes in cell membranes by fluorescence polarization-resolved imaging<\/i>
\nBiophys. J.<\/b>\u00a0101, pp. 468\u2013476 (2011)<\/p>\n

P. Refregier, M.\u00a0Roche, S. Brasselet
\nPrecision analysis in polarization-resolved second harmonic generation microscopy<\/i>
\nOpt. Lett. Vol.<\/b>\u00a036 (11), pp. 2149-2151 (2011)<\/p>\n

S. Brasselet
\nPolarization resolved nonlinear microscopy\u00a0: application to structural molecular and biological imaging<\/i>
\nAdvances in Optics and Photonics<\/b>\u00a03, pp. 205\u2013271 (2011)<\/p>\n

2010<\/strong>
\nF. Munhoz, H. Rigneault, S. Brasselet
\nHigh Order Symmetry Structural Properties of Vibrational Resonances Using Multiple-Field Polarization Coherent Anti-Stokes Raman Spectroscopy Microscopy<\/i>
\nPhys Rev Lett.<\/b>\u00a0105, 123903 (2010)<\/p>\n

D. A\u00eft-Belkacem, A. Gasecka, F. Munhoz, S. Brustlein, and S. Brasselet
\nInfluence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging<\/i>
\nOpt. Express<\/b>\u00a018 (14) 14859-14870 (2010)<\/p>\n

S. Brasselet
\nSecond Harmonic Generation microscopy in molecular crystalline nano-objects<\/i>
\nNonlinear Optics<\/b>, Quantum Optics NLOQO, 40 (1-4), pp 83-94 (2010)<\/p>\n

A. Gasecka, L-Q. Dieu, D. Bruehviler, S. Brasselet
\nProbing molecular order in zeolite L inclusion compounds using two-photon fluorescence polarimetric microscopy<\/i>
\nJ. Phys. Chem. B<\/b>\u00a0114 (12), pp 4192\u20134198 (2010)<\/p>\n

P. Sch\u00f6n, M.\u00a0Behrndt, D. Ait-Belkacem, H. Rigneault, S. Brasselet
\nPolarization and Phase Pulse Shaping applied to Structural Contrast in Nonlinear Microscopy Imaging<\/i>
\nPhys. Rev. A<\/b>\u00a081, 013809 (2010)<\/p>\n

2009<\/strong>
\nF. Munhoz, S. Brustlein, D. Gachet, F. Billard, S. Brasselet, H. Rigneault
\nRaman depolarization ratio of liquids probed by linear polarization Coherent Anti-Stokes Raman spectroscopy<\/i>
\nJournal of Raman Spectroscopy<\/b>, vol. 40, (7), 775-780 (2009)<\/p>\n

A. Gasecka, T.-J. Han, C. Favard, B.R. Cho, S. Brasselet
\nQuantitative imaging of molecular order in lipid membranes using two-photon fuorescence polarimetry<\/i>
\nBiophys J.<\/b>\u00a097 (10) 2854-2862 (2009)<\/p>\n","protected":false},"author":13,"featured_media":2082,"parent":1742,"menu_order":16,"template":"","thematique":[24],"class_list":{"0":"post-1890","1":"mosaic","2":"type-mosaic","3":"status-publish","4":"has-post-thumbnail","6":"entry"},"acf":[],"lang":"en","translations":{"en":1890,"fr":1340},"pll_sync_post":[],"_links":{"self":[{"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/mosaic\/1890","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/mosaic"}],"about":[{"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/types\/mosaic"}],"author":[{"embeddable":true,"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/users\/13"}],"version-history":[{"count":12,"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/mosaic\/1890\/revisions"}],"predecessor-version":[{"id":5254,"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/mosaic\/1890\/revisions\/5254"}],"up":[{"embeddable":true,"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/mosaic\/1742"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/media\/2082"}],"wp:attachment":[{"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/media?parent=1890"}],"wp:term":[{"taxonomy":"thematique","embeddable":true,"href":"https:\/\/www.fresnel.fr\/wp\/wp-json\/wp\/v2\/thematique?post=1890"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}