using wavefront imaging
Principal investigators: Serge Monneret, Guillaume Baffou, Julien Savatier
keywords: lateral shearing interferometry, wavefront microscopy
Quantitative phase imaging (QPI) techniques are now conventionally used in microscopy for measuring specific properties of semi-transparent samples without any labelling. By placing a wavefront imaging system on the exit image plane of a microscope, we measure the complex field spatial distribution in this plane, and then (...)
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Research Topics
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Quantitative phase microscopy for Biology
18 July 2016 -
Wavefront shaping in scattering media
18 July 2016for in-depth nonlinear imaging in tissues
Principal investigators: Sophie Brasselet
keywords:in-depth two-photon and coherent Raman scattering (CRS) imaging, wavefront shaping, adaptive optics
The potential of label-free imaging techniques based on nonlinear optics presents limits in turbid or heterogeneous media like biological tissues. Thanks to recent advances in wavefront control technologies (spatial light modulators based on liquid crystals, deformable mirrors), imaging in (...) -
Polarized microscopy
18 July 2016from single molecules to tissues
Principal investigators: Sophie Brasselet
keywords: polarized fluorescence, polarized super-resolution imaging, polarized nonlinear microscopy
Polarization is an important property of light that is often ignored in light-matter interaction, in particular for bio-imaging applications. We develop methodologies for optical microscopy that exploit the properties of light polarization by a control of the incident or detected polarization states. This (...) -
Microspheres and single molecule detection
18 July 2016Principal investigator: Jerome Wenger
Complex optical instrumentation, and/or expensive nanofabrication can hinder the progress of photonic technologies. Here we demonstrate that dielectric microspheres offer a relevant cost-effective alternative to more advanced lenses and objectives.
Beating the diffraction barrier with microspheres
Commercially available microspheres under focused Gaussian illumination can achieve three-axis optical confinement below the diffraction limit, with an (...) -
Nanoaperture enhanced fluorescence
18 July 2016Metal nanoapertures and zero-mode waveguides
Principal investigator: Jerome Wenger
Milling a nanometer-size aperture in a metallic film is an intuitive way to fabricate nanophotonic devices. Although the concept appears very simple, such apertures (also known as zero-mode waveguides) exhibit attractive properties for biophotonics: localization of excitation light strong isolation from emission produced by species located outside the aperture enhancement in the fluorescence signal (...) -
Plasmonic enhanced energy transfer
18 July 2016Accelerating energy transfer between molecules with optical nanoantennas
Principal investigator: Jerome Wenger
Energy transfer between molecules is promoted when they are set in an environment that confines light. The energy transfer between molecules is an essential phenomenon for photosynthesis, photovoltaics and biotechnology. Now, thanks to the work of the Institut Fresnel Institute, energy transfer between molecules can be controlled and enhanced with optical structures etched at (...) -
Nanoantenna enhanced fluorescence
18 July 2016Antennas in nanobiophotonics
Principal investigator: Jerome Wenger
Nanophotonics can improve single molecule optical detection beyond the diffraction limit. The main goal is to tailor the electromagnetic environment by the use of an optical antenna to concentrate the light on a tiny spot and simultaneously enhance the molecular emission. Single molecule spectroscopy techniques, FRET and FCS can greatly benefit from photonic nanoantennas to enter a new dimension of higher sensitivities (...) -
Overview
1 April 2016Morphogenesis Studying the organization of living matter Studying the mechanisms underlying shape changes at the molecular, cellular and tissue scales Nanobiophotonics Nanoantenna enhanced fluorescence
Plasmonic enhanced energy transfer
Nanoaperture enhanced fluorescence
Microspheres & single molecule detection
Label-free molecular diagnosis and biosensor
Protein interactions with FCS and FRET Using optical antennas (...) -
Thermoplasmonics
1 April 2016using gold nanoparticles as nanosources of heat
Principal investigator: Guillaume Baffou
keywords: nanoplasmonics, gold nanoparticles, thermal effects, optical and thermal imaging
Our research activities stand at the frontiers between nanooptics and thermodynamics. We investigate thermal-induced processes at the nano and micro scales using photothermally excited gold nanoparticles [13].
I - Experimentally
Our major contribution to this field of research (named thermoplasmonics) (...)
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