Fabian Voigt, Engert Lab - Harvard University

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Tuesday, September 12th, at 9:30 a.m. in room Pierre Cotton of Institut Fresnel, Fabian VOIGT, from the Engert lab at Harvard University, USA, will give a seminar entitled Expanding the optical bag of tricks for neuroscience.

Abstract : Seeing is believing and thus, optical imaging techniques are extremely useful to study brain structure and function. I will present two projects aimed at providing the neuroscience community with better imaging instrumentation. In the first part, I will introduce the mesoSPIM, an open-source light-sheet microscope that is optimized for fast imaging of large cleared tissue samples at 5-7 µm isotropic resolution. Since 2015, the mesoSPIM evolved from a crude prototype into a highly capable instrument and we built a global community around it. In the second part, I will talk about a recent project that takes inspiration from scallops and astronomy to build novel multi-immersion microscope objectives that are well suited for imaging cleared samples. These objectives combine long working distances (>1 cm), large FOVs (>1 mm), high numerical aperture (currently up to 1.08) with diffraction-limited resolution in any homogeneous medium ranging from air to the typical high-index immersion liquids used for imaging cleared tissue. They are especially well suited to augment low-to-mid resolution mesoSPIM overviews with high-resolution datasets.

Biography : Fabian Voigt studied Interdisciplinary Sciences at ETH Zurich and received his PhD in 2019 from the University of Zurich. During his PhD work in the laboratory of Fritjof Helmchen, he developed the mesoscale selective plane illumination (mesoSPIM) Initiative, a global open science project aiming to provide the imaging community with better light-sheet microscopes for imaging cm-sized cleared tissue samples. Since Fall 2021, Fabian Voigt is a HFSP postdoctoral fellow in the lab of Florian Engert at Harvard University and working on improving whole-brain imaging techniques in larval zebrafish.

Contact :
Fabian F. Voigt, Department of Molecular and Cellular Biology, Harvard University - fvoigt@fas.harvard.edu

Invitation : Imaging Theme, Loic Le Goff & Thomas Chaigne