Photoacoustic imaging of neuronal activity in mice

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MASTER Internship

Research program :
The study of large scale neuronal circuits throughout the brain is currently one of the biggest challenge in neurobiology. Non invasive imaging of neuronal activity with single cell resolution is however currently limited to shallow depths, due to prominent light scattering beyond one millimeter. Photoacoustic imaging, a fascinating technique relying on ultrasound generation upon the absorption of a light pulse, has been developed to overcome this issue, enabling to probe optical absorption contrast at large depths in biological tissue.
The project aims at developing an all-optical photoacoustic imaging setup to non-invasively access neuronal activity at large depths (> 2 mm) in the mouse brain.
To provide a sufficient detection bandwidth and hence achieve quasi cellular resolution, the acoustic field will be optically detected using custom-made Fabry-Pérot cavities. Wide-field interrogation strategies will be investigated to yield the high temporal resolution required for neuronal activity imaging. We will apply these techniques to perform calcium imaging in mice.

Collaborations : As dealing with advanced optical fabrication and neurobiology, this project will be carried out in close collaboration with other groups from Marseille (RCMO group at Fresnel Institute, Cossart group at Inmed).

Place : Institut Fresnel (host Lab) & INMED, Marseille

Time : Flexible duration of the internship (> 3 months) & flexible period

Supervisor :