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Quantitative phase microscopy for Biology

using wavefront sensing

Principal investigators: Serge Monneret, Guillaume Baffou, Julien Savatier

keywords: lateral shearing interferometry, wavefront microscopy

Quantitative phase imaging (QPI) techniques are now conventionally used in microscopy for measuring specific properties of semi-transparent samples without any labelling. By placing a wavefront sensor on the exit image plane of a microscope, we measure the complex field spatial distribution in this plane, and then retrieve the quantitative optical path difference (OPD) of the observed sample.

The QPI technique we use is a wavefront imaging technique based on Quadri-Wave Lateral Shearing Interferometer (QLSI). It consists of a 2D-grating placed at a millimetric distance from a regular camera. QLSI can reach the diffraction limit, and it is achromatic, and thus compatible with the white-light illumination pathway of conventional wide-field microscopes.

In the last years, we extended the technique to new applications, where different physical phenomena produced a given sample-induced change in the phase of the exit optical beam that modulates the incident wavefront. More precisely, we used direct refractive-induced, thermal-induced, and resonant vibrational-induced OPD variations to study physical or biological samples, the retardance, temperature distribution of complex patterns of nanostructures, and Raman spectra of polystyrene beads.

Application area

Quantitative phase microscopy on living cells, optical waveguides, laser damage sites
Optical profilometry on etched surfaces, laser damage craters
Dry mass determination of cells / Cell cycle follow-up
Quantitative phase imaging with nonlinear microscopy (CARS)
Refractive index and thermo-refractive index (dn/dT) measurement of liquids
Phase tomography
Optical retardance imaging / specific imaging of birefringent samples
Temperature microscopy
3D localization of metallic nanoparticles

Publications

2023
Dry mass photometry of single bacteria using quantitative wavefront microscopy
M. Bénéfice, A. Gorlas, B. Marthy, V. Da Cunha, P. Forterre, A. Sentenac, P. C. Chaumet, G. Baffou
Biophysical Journal 122, 1-14 (2023)
Wavefront microscopy using quadriwave lateral shearing interferometry: from bioimaging to nanophotonics
G. Baffou
ACS Photonics 10, 322 (2023)
2022
Cross-grating phase microscopy (CGM): In-silico experiment (insilex) algorithm, noise and accuracy
B. Marthy, G. Baffou
Optics Communications 521, 128577 (2022)
Biomass measurements of single neurites in vitro using optical wavefront microscopy
L. Durdevic, A. Relaño Ginés, A. Roueff, G. Blivet, G. Baffou
Biomedical Optics Express 13, 6550 (2022).
Life at high temperature observed in vitro upon laser heating of gold nanoparticles
C. Molinaro, M. Bénéfice, A. Gorlas, V. Da Cunha, H. M. L. Robert, R. Catchpole, L. Gallais, P. Forterre, G. Baffou
Nature Communications 13, 5342 (2022).
Cross-grating phase microscopy (CGM): In-silico experiment (insilex) algorithm, noise and accuracy
B. Marthy, G. Baffou
Optics Communications 521, 128577 (2022).
2021
Quantitative phase microscopy using quadriwave lateral shearing interferometry (QLSI): principle, terminology, algorithm and grating shadow description.
G. Baffou
J. Phys. D: Appl. Phys. 54, 294002 (2021).
2019
Quantitative phase imaging of adherent mammalian cells: a comparative study
Allier, C; Herve, L; (...); Morales, S
Biomed. Opt. Exp. 10, 2768-2783 (2019).
Wavefront sensing applied to determine the temperature dependence of the refractive index of liquids
Radhakrishnan, R; Gallais, L and Monneret, S
Appl. Opt. 58, 3646-3651 (2019).
Large field-of-view phase and fluorescence mesoscope with microscopic resolution
de Kernier, I; Ali-Cherif, A; (...); Blandin, P; Rongeat, N.; Cioni, O.; Morales, S.; Savatier, S.; Monneret, S.; Blandin, P.
J. Biomed. Opt. 24, 036501 (2019).
2018
Quantitative retardance imaging by means of quadri-wave lateral shearing interferometry for label-free fiber imaging in tissues
Aknoun, S; Aurrand-Lions, M; Wattellier, B.; Monneret, S
Opt. Commun. 422, 17-27 (2018).
2017
Laser-induced birefringence measurements by quantitative polarized-phase microscopy
Doualle, T; Olle, A; Cormont, P.; Monneret, S.; Gallais, L
Optics Letters 42(8), 1616-1619 (2017).
2016
Time-resolved quantitative phase microscopy of laser-material interactions using a wavefront sensor
L. Gallais, S. Monneret
Optics Letters 41(14), 3245-3248 (2016).
2015
Living cells dry mass measurements using Quantitative Phase Imaging with Quadri Wave Lateral Shearing Interferometry. An accuracy and sensitivity discussion
S. Aknoun, J. Savatier, P. Bon, F. Galland, L. Abdeladim, B. Wattellier, S. Monneret
Journal of Biomedical Optics 20(12), 126009 (2015).
Quantitative phase imaging applied to laser damage detection and analysis
D.-B. Douti, M. Chrayteh, S. Aknoun, T. Doualle, C. Hecquet, S. Monneret, L. Gallais
Applied Optics 28 (54), 8375-8382 (2015)
Three dimensional nanometer localization of nanoparticles to stabilize super-resolution microscopy
P. Bon, N. Bourg, S. Lécart, S. Monneret, E. Fort, J. Wenger, S. Lévêque-Fort
Nature Communications 6, 7764 (2015)
Quantitative Retardance Imaging of biological samples using Quadri-Wave Lateral Shearing Interferometry
S. Aknoun, P. Bon, J. Savatier, B. Wattellier, S. Monneret
Optics Express 23 (12), 16383-16406 (2015).
2013
Photo-induced heating of nanoparticle arrays
G. Baffou, P. Berto, E. Bermudez, R. Quidant, S. Monneret, J. Polleux, H. Rigneault
ACS Nano 7 (11), 6478-6488 (2013)
Three-dimensional temperature imaging around a gold microwire
P. Bon, N. Belaid, D. Lagrange, C. Bergaud, H. Rigneault, S. Monneret, G. Baffou
Applied Physics Letters 102, 244103 (2013)
Wide-field vibrational phase imaging in an extremely folded box-coherent anti-Stokes Raman scattering geometry
P. Berto, A. Jesacher, C. Roider, S. Monneret, H. Rigneault, M. Ritsch-Marte
Optics Letters 38 (5), 709-711 (2013).
2012
Wide-field vibrational phase imaging
P. Berto, D. Gachet, P. Bon, S. Monneret, H. Rigneault
Physical Review Letters 109, 093902 (2012).
Imaging the Gouy phase shift in photonic jets with a wavefront sensor
P. Bon, B. Rolly, N. Bonod, J. Wenger, B. Stout, S. Monneret, H. Rigneault
Optics Letters 37 (17), 3531-3533 (2012).
Non-iterative boundary-artifact free wavefront reconstruction from its derivatives
P. Bon, S. Monneret, B. Wattellier
Applied Optics 51 (23), 5698-5704 (2012)
Optical detection and measurement of living cell morphometric features with single-shot quantitative phase microscopy
P. Bon, J. Savatier, M. Merlin, B. Wattellier, S. Monneret
Journal of Biomedical Optics 17 (7), 076004 (2012)
Modeling quantitative phase image formation under tilted illuminations
P. Bon, B. Wattellier, S. Monneret
Optics Letters 37 (10), 1718-1720 (2012)
Selected in Virtual Journal for Biomedical Optics 7 (7), 2012
Tomographic diffractive microscopy with a wavefront sensor
Y. Ruan, P. Bon, E. Mudry, G. Maire, P. Chaumet, H. Giovannini, K. Belkebir, A. Talneau, B. Wattellier, S. Monneret, A. Sentenac
Optics Letters 37 (10), 1631-1633 (2012)
Selected in Virtual Journal for Biomedical Optics 7 (7), 2012
Quantitative absorption spectroscopy of nano-objects
P. Berto, E. Bermúdes Ureña, P. Bon, R. Quidant, H. Rigneault, G. Baffou
Physical Review B 86, 165417 (2012)
2009
Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells
P. Bon, G. Maucort, B. Wattellier, S. Monneret
Optics Express 17 (15), 13080-13094 (2009)
Selected in Virtual Journal for Biomedical Optics 4 (9), 2009

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