Etude du vivant (biologie et biomedical)
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Quantitative phase microscopy
using wavefront sensing
Principal investigators : Serge Monneret, Julien Savatier
keywords : lateral shearing interferometry, phase contrast, wavefront sensing, microscopy
Quantitative phase imaging techniques are now conventionally used in microscopy for measuring specific properties of semi-transparent samples without any labelling. By placing a wavefront sensor on the exit image plane of a microscope, we measure the complex field spatial distribution in this plane, and then retrieve the quantitative optical path difference (OPD) of the observed sample. Most of works presented in this page were developed in a close collaboration with a company (Phasics SA, Saint-Aubin, France).
The wavefront sensor we use is a commercial Quadri-Wave Lateral Shearing Interferometer (QWLSI) provided by Phasics SA. It is an efficient tool for measuring phase gradients of optical beams along two perpendicular directions. Post-processing integration then allows obtaining the complete phase spatial distribution of the beam with a high spatial resolution (diffraction-limited). Moreover, QWLSI is achromatic, and thus compatible with the white-light illumination pathway of conventional wide-field microscopes.
In the last years, we extended the technique to new applications, where different physical phenomena produced a given sample-induced change in the phase of the exit optical beam that modulates the incident wavefront. More precisely, we used direct refractive-induced OPD, thermal-induced OPD, and resonant vibrational-induced OPD to produce phase contrast images of physical or biological samples, temperature distribution of complex patterns of nanostructures, and Raman spectra of polystyrene beads, respectively. We are now developing the technique to reach 3D resolution, but also to focus on optical retardance distribution inside observed samples.
Application area
Quantitative phase microscopy on living cells, optical waveguides, laser damage sites |
Measurement of photo-induced changes in materials (laser damage, local temperature) |
Optical profilometry on etched surfaces, laser damage craters |
Dry mass determination of cells / Cell cycle follow-up |
Quantitative phase imaging with nonlinear microscopy (CARS) |
Refractive index and thermo-refractive index (dn/dT) measurement of liquids |
Phase tomography |
Optical retardance imaging / specific imaging of birefringent samples |
Temperature microscopy (see "thermoplasmonics" page) |
3D localization of metallic nanoparticles |
Publications
2019
S. Khadir,* P. Chaumet, G. Baffou, A. Sentenac
Journal of the Optical Society of America A 36, 478-484 (2019).
2018
S. Khadir, P. Bon, D. Vignaud, E. Galopin, N. McEvoy, D. McCloskey, S. Monneret, G. Baffou
ACS Photonics 4, 3130–3139 (2017).
2016
L. Gallais, S. Monneret
Optics Letters 41(14), 3245-3248 (2016).
2015
S. Aknoun, J. Savatier, P. Bon, F. Galland, L. Abdeladim, B. Wattellier, S. Monneret
Journal of Biomedical Optics 20(12), 126009 (2015).
D.-B. Douti, M. Chrayteh, S. Aknoun, T. Doualle, C. Hecquet, S. Monneret, L. Gallais
Applied Optics 28 (54), 8375-8382 (2015)
P. Bon, N. Bourg, S. Lécart, S. Monneret, E. Fort, J. Wenger, S. Lévêque-Fort
Nature Communications 6, 7764 (2015)
S. Aknoun, P. Bon, J. Savatier, B. Wattellier, S. Monneret
Optics Express 23 (12), 16383-16406 (2015).
2013
G. Baffou, P. Berto, E. Bermudez, R. Quidant, S. Monneret, J. Polleux, H. Rigneault
ACS Nano 7 (11), 6478-6488 (2013)
P. Bon, N. Belaid, D. Lagrange, C. Bergaud, H. Rigneault, S. Monneret, G. Baffou
Applied Physics Letters 102, 244103 (2013)
P. Berto, A. Jesacher, C. Roider, S. Monneret, H. Rigneault, M. Ritsch-Marte
Optics Letters 38 (5), 709-711 (2013).
2012
P. Berto, D. Gachet, P. Bon, S. Monneret, H. Rigneault
Physical Review Letters 109, 093902 (2012).
P. Bon, B. Rolly, N. Bonod, J. Wenger, B. Stout, S. Monneret, H. Rigneault
Optics Letters 37 (17), 3531-3533 (2012).
P. Bon, S. Monneret, B. Wattellier
Applied Optics 51 (23), 5698-5704 (2012)
P. Bon, J. Savatier, M. Merlin, B. Wattellier, S. Monneret
Journal of Biomedical Optics 17 (7), 076004 (2012)
P. Bon, B. Wattellier, S. Monneret
Optics Letters 37 (10), 1718-1720 (2012)
Selected in Virtual Journal for Biomedical Optics 7 (7), 2012
Y. Ruan, P. Bon, E. Mudry, G. Maire, P. Chaumet, H. Giovannini, K. Belkebir, A. Talneau, B. Wattellier, S. Monneret, A. Sentenac
Optics Letters 37 (10), 1631-1633 (2012)
Selected in Virtual Journal for Biomedical Optics 7 (7), 2012
P. Berto, E. Bermúdes Ureña, P. Bon, R. Quidant, H. Rigneault, G. Baffou
Physical Review B 86, 165417 (2012)
2009
P. Bon, G. Maucort, B. Wattellier, S. Monneret
Optics Express 17 (15), 13080-13094 (2009)
Selected in Virtual Journal for Biomedical Optics 4 (9), 2009