Biologie
Accueil › Recherche › Imagerie › Biologie › Non-linear optics for label-free microscopy
Non-linear optics for label-free microscopy
Principal investigators : Hervé Rigneault, Sandro Heuke, Sophie Brasselet, Julien Duboisset
keywords : 2-photon fluorescence, second and third harmonic generation, coherent Raman scattering, pump-probe, nonlinear imaging, molecular spectroscopy
We are interested in how nonlinear optical contrast mechanisms can provide novel molecular information in microscopy and micro-spectroscopy. These modalities take advantage of label free capabilities, deep penetration into scattering samples, as well as high order (wave mixing) light matter interaction properties. Our work aims at providing insight into
- the fundamental nonlinear light matter interaction and the construction of the generated light fields under tight focusing conditions and
- the molecular properties that can be addressed and readout through these nonlinear processes.
On the instrumentation side, we develop multimodal nonlinear microscopy and micro-spectroscopy technologies using ultra-short laser pulses (from 10 fs to ps), possibly synchronized (using electronic synchronization or OPOs). Special attention is given to coherent Raman scattering modalities such as coherent anti-Stokes Raman scattering (CARS ) and stimulated Raman scattering (SRS) for label-free vibrational microscopy and micro-spectroscopy.
Multimodal nonlinear microscopy is used to address a variety of problems such as :
- Drug penetration in tissues (cosmetic, pharmaceutical and medical applications)
- Architecture of collagen fibers in tissue
- Lipid metabolism (cancer tissues)
- Myelin organization (neurobiology)
- Tissue morphology (developmental biology, cancer tissues)
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Publications
2023
E. M. Fantuzzi, S. Heuke, S. Labouesse, D. Gudavičius, R. Bartels, A. Sentenac, and H. Rigneault, Nat. Photon. 48, 4113-4116 (2023).
10.1038/s41566-023-01294-x
S. Heuke, C. Silva Martins, R. André, L. LeGoff, and H. Rigneault, Opt. Lett. : Photonics 48, 4113-4116 (2023).
10.1364/OL.496071
H. Rigneault, S. Heuke, Photoniques 121, 52-56, 2023.
10.1051/photon/202312152
C. Scotté, F. Galland, and H. Rigneault, Journal of Physics : Photonics 5, 035003 (2023).
10.1088/2515-7647/acc70b
S. Heuke, and H. Rigneault, Nature Communications 14, 3337 (2023).
10.1038/s41467-023-38941-4
S. Shivkumar, D. Ranann, S. Metais, S. Suresh, N. Forget, R. Bartels, D. Oron, and H. Rigneault, Physical Review Applied 19, 054075 (2023).
10.1103/PhysRevApplied.19.054075
R. Appay, B. Sarri, S. Heuke, S. Boissonneau, C. Liu, E. Dougy, L. Daniel, D. Scavarda, H. Dufour, D. Figarella-Branger, and H. Rigneault, J. Phys. Chem. B 127, 3624-3631 (2023).
10.1021/acs.jpcb.3c01156
S. Heuke, X. Audier, H. Rigneault, Optics Letters 48, 423-426 (2023).
10.1364/OL.467514
2022
C. Grand, C. Scotté, and H. Rigneault, Analytical Chemistry 94, 16632-16637 (2022).
10.1021/acs.analchem.2c02680
D. R. Smith, S. Shivkumar, J. Field, J. W. Wilson, H. Rigneault, and R. A. Bartels, Optics Letters 47, 5841-5844 (2022).
10.1364/OL.467970
2021
S. Heuke, I. Rimke, B. Sarri, P. Gasecka, R. Appay, L. Legoff, P. Volz, E. Büttner, and H. Rigneault, Biomed. Opt. Express 12, 7780-7789 (2021).
doi.org/10.1364/BOE.446348
B. Sarri, R. Appay, S. Heuke, F. Poizat, F. Franchi, S. Boissonneau, F. Caillol, H. Dufour, D. Figarella-Branger, M. Giovaninni, and H. Rigneault,Translational Biophotonics n/a e202000020 (2021).
doi.org/10.1002/tbio.202000020
S. Heuke, H. Rigneault, and A. Sentenac, Optics Express 29, 4230-4239 (2021).
doi.org/10.1364/OE.416290
S. Heuke, and H. Rigneault, Optics Express 29, 3985-3995 (2021).
doi.org/10.1364/OE.414972
2020
X. Audier, S. Heuke, P. Volz, I. Rimke, and H. Rigneault, APL Photonics 5, 011101 (2020).
doi.org/10.1063/1.5129212
S. Heuke, A. Lombardini, E. Büttner, and H. Rigneault, Optics Express 28, 29619-29630 (2020).
doi.org/10.1364/OE.400298
A. Lombardini, P. Berto, J. Duboisset, E. R. Andresen, S. Heuke, E. Büttner, I. Rimke, S. Vergnole, V. Shinkar, P. de Bettignies, and H. Rigneault, Optics Express 28, 14502 (2020).
doi.org/10.1364/OE.390381
C. Scotté, S. Sivankutty, R. A. Bartels, and H. Rigneault, Optics Letters 45, 5567-5570 (2020)
doi.org/10.1364/OL.400151
X. Audier, N. Forget, and H. Rigneault, Optics Express 28, 15505-15514 (2020).
doi.org/10.1364/OE.390850
S. Heuke, S. Sivankutty, C. Scotte, P. Stockton, R. A. Bartels, A. Sentenac, and H. Rigneault, Optica 7, 417-424 (2020).
A. Lombardini, P. Berto, J. Duboisset, E. R. Andresen, S. Heuke, E. Büttner, I. Rimke, S. Vergnole, V. Shinkar, P. de Bettignies, and H. Rigneault, Optics Express 28, 14490-14502 (2020)
2019
B. Sarri, R. Canonge, X. Audier, V. Lavastre, G. Pénardier, J. Alie, H. Rigneault, J Raman Spectrosc. (2019)
D. Raanan, X. Audier, S. Shivkumar, M. Asher, M. Menahem, O. Yaffe, N. Forget, H. Rigneault, and D. Oron, Optics Letters 44, 5153-5156 (2019)
B. Sarri, F. Poizat, S. Heuke, J. Wojak, F. Franchi, F. Caillol, M. Giovannini, and H. Rigneault, Biomed. Opt. Express 10, 5378-5384 (2019)
B. Sarri, X. Chen, R. Canonge, S. Grégoire, F. Formanek, J.-B. Galey, A. Potter, T. Bornschlögl, and H. Rigneault, Journal of Controlled Release 308, 190-196 (2019)
2018
S. Heuke, B. Sarri, A. Lombardini, X. Audier, and H. Rigneault, Optics Letters 43, 4763-4766 (2018)
S. Heuke, B. Sarri, X. Audier, and H. Rigneault, Optics Letters 43, 3582-3585 (2018)
2017
P. Berto, C. Scotté, F. Galland, H. Rigneault, and H. B. de Aguiar, Optics Letters 42, 1696-1699 (2017)
X.Audier, N. Balla, and H. Rigneault, Optics Letters 42, 294-297 (2017)
2016
Mytskaniuk, V., Bardin, F., Boukhaddaoui, H., Rigneault, H., Tricaud, N.
J. Vis. Exp. 113, e54262, doi:10.3791/54262 (2016)
C. Cleff, A. Gasecka, P. Ferrand, H. Rigneault, S. Brasselet, and J. Duboisset
Nat Commun 7, 11562 (2016), doi:10.1038/ncomms1156
S. Saint-Jalm, E. R. Andresen, A. Bendahmane, A. Kudlinski, and H Rigneault
Opt. Lett. 41, 115-118 (2016)
X. Chen, P. Gasecka, F. Formanek, J. B. Galey, and H. Rigneault
British Journal of Dermatology (2016) - DOI : 10.1111/bjd.14292
2015
X. Chen, S. Grégoire, F. Formanek, J.-B. Galey, H. Rigneault
Journal of Controlled Release 200, 78-86 (2015)
2014
S. Saint-Jalm, E. R. Andresen, P. Ferrand, A. Bendahmane, A. Mussot, O. Vavincq, G. Bouwnmans, A. Kudlinski, H. Rigneault
Journal of Biomedical Optics 19, 086021 (2014)
S. Saint-Jalm, P. Berto, L. Jullien, E. R. Andresen, and H. Rigneault
Journal of Raman Spectroscopy 45, 515-520 (2014)
P. Berto, E. R. Andresen, H. Rigneault
Phys. Rev. Lett 112, 053905 (2014)
2013
P. Berto, A. Jesacher, C. Roider, S. Monneret, H. Rigneault, and M. Ritsch-Marte
Optics Letters 38, 709-711 (2013)
2012
H. Rigneault, E. Andresen
Nat. Phot 6, 802-803 (2012)
P. Berto, D. Gachet, P. Bon, S. Monneret and H. Rigneault
Phys. Rev. Lett. 109, 093902 (2012)
F. Munhoz, S. Brustlein, R. Hostein, P. Berto, S. Brasselet and H. Rigneault
Journal of Raman Spectroscopy 43, 419–424 (2012)
2011
D. Gachet, H. Rigneault
J. Opt. Soc Am. A 28, pp. 2531-2539 (2011)
D. Gachet, H. Rigneault
J. Opt. Soc Am. A 28, pp.2519-2530 (2011)
S. Brustlein ; D. Gachet ; F. Billard ; H. Rigneault
Journal of Biomedical Optics 16, 086006 (2011)
E.R. Andresen, P. Berto, H. Rigneault
Opt Lett 36, 2387-2389 (2011)
S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet and H Rigneault
J. Biomed. Opt. 16, 021106 (2011)
S. Michel, A. Courjaud, E. Mottay, J. Dudley, C. Finot, H. Rigneault
J. Biomed. Opt. 16, 021108 (2011)
2010
F. Munhoz, H. Rigneault, S. Brasselet
Phys. Rev. Lett. 105, 123903 (2010)
D. Gachet, S. Brustlein, H. Rigneault
Phys. Rev. Lett. 104, pp.213905 (2010)
2009
David Gachet, Franck Billard, and Hervé Rigneault
Opt. Lett. 34, 1789-1791 (2009)
F. Billard, D. Gachet, and H. Rigneault
J. Opt. Soc. Am. B 26, 1295-1309 (2009)
F. Munhoz, S. Brustlein, D. Gachet, F. Billard, S. Brasselet and H. Rigneault
Journal of Raman Spectroscopy 40, 775-780 (2009)
2008
D. Gachet, F. Billard, H. Rigneault
J. Opt. Soc. Am. B 25, 1655-1666 (2008)
D. Gachet, F. Billard, and H. Rigneault
Phys. Rev. A 77, 061802(R) (2008)
2007
D. Gachet, F. Billard, N. Sandeau and H. Rigneault
Optics Express 15 (16), 10408-10420 (2007)
N. Djaker, P.-F. Lenne, D. Marguet, A. Colonna, C. Hadjur, H. Rigneault
Nuclear Instruments and Methods in Physics Research A 571, 177–181 (2007)
2006
N. Djaker, D. Marguet, H. Rigneault
Médecine-Sciences 22 (10), pp853-858 (2006)
D. Gachet, N. Sandeau, H. Rigneault
J. Europ. Opt. Soc. Rap. Com 1, 6013 (2006)
N. Djaker, D. Gachet, N. Sandeau, P.-F. Lenne, and H. Rigneault
Applied Optics 45 (27), 7005-7011 (2006)