Etude du vivant (biologie et biomedical)

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Non-linear optics for label-free microscopy

Principal investigators : Hervé Rigneault, Sophie Brasselet, Julien Duboisset

keywords : 2-photon fluorescence, second and third harmonic generation, coherent Raman scattering, pump-probe, nonlinear imaging, molecular spectroscopy

We are interested in how nonlinear optical contrast mechanisms can provide novel molecular information in microscopy and micro-spectroscopy. These modalities take advantage of label free capabilities, deep penetration into scattering samples, as well as high order (wave mixing) light matter interaction properties. Our work aims at providing insight into

  • the fundamental nonlinear light matter interaction and the construction of the generated light fields under tight focusing conditions and
  • the molecular properties that can be addressed and readout through these nonlinear processes.

On the instrumentation side, we develop multimodal nonlinear microscopy and micro-spectroscopy technologies using ultra-short laser pulses (from 10 fs to ps), possibly synchronized (using electronic synchronization or OPOs). Special attention is given to coherent Raman scattering modalities such as coherent anti-Stokes Raman scattering (CARS ) and stimulated Raman scattering (SRS) for label-free vibrational microscopy and micro-spectroscopy.

Multimodal nonlinear microscopy is used to address a variety of problems such as :

  • Drug penetration in tissues (cosmetic, pharmaceutical and medical applications)
  • Architecture of collagen fibers in tissue
  • Lipid metabolism (cancer tissues)
  • Myelin organization (neurobiology)
  • Tissue morphology (developmental biology, cancer tissues)

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Publications

2018
Dual-focus stimulated Raman scattering microscopy : a concept for multi-focus scaling
S. Heuke, B. Sarri, A. Lombardini, X. Audier, and H. Rigneault, Optics Letters 43, 4763-4766 (2018)
Simultaneous dual-channel stimulated Raman scattering microscopy demultiplexed at distinct modulation frequencies
S. Heuke, B. Sarri, X. Audier, and H. Rigneault, Optics Letters 43, 3582-3585 (2018)
2017
Programmable single-pixel-based broadband stimulated Raman scattering
P. Berto, C. Scotté, F. Galland, H. Rigneault, and H. B. de Aguiar, Optics Letters 42, 1696-1699 (2017)
Pump-probe micro-spectroscopy by means of an ultra-fast acousto-optics delay line
X.Audier, N. Balla, and H. Rigneault, Optics Letters 42, 294-297 (2017)
2016
Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
Mytskaniuk, V., Bardin, F., Boukhaddaoui, H., Rigneault, H., Tricaud, N.
J. Vis. Exp. 113, e54262, doi:10.3791/54262 (2016)
Direct imaging of molecular symmetry by coherent anti-stokes Raman scattering
C. Cleff, A. Gasecka, P. Ferrand, H. Rigneault, S. Brasselet, and J. Duboisset
Nat Commun 7, 11562 (2016), doi:10.1038/ncomms1156
Nonlinear femtosecond pump-probe spectroscopy using a power encoded soliton delay line
S. Saint-Jalm, E. R. Andresen, A. Bendahmane, A. Kudlinski, and H Rigneault
Opt. Lett. 41, 115-118 (2016)
In vivo single human sweat gland activity monitoring using coherent anti-Stokes Raman scattering and two-photon excited auto-fluorescence microscopy
X. Chen, P. Gasecka, F. Formanek, J. B. Galey, and H. Rigneault
British Journal of Dermatology (2016) - DOI : 10.1111/bjd.14292
2015
Quantitative 3D molecular cutaneous absorption in human skin using label free nonlinear microscopy
X. Chen, S. Grégoire, F. Formanek, J.-B. Galey, H. Rigneault
Journal of Controlled Release 200, 78-86 (2015)
2014
Fiber-based ultrashort pulse delivery for nonlinear imaging using high energy solitons
S. Saint-Jalm, E. R. Andresen, P. Ferrand, A. Bendahmane, A. Mussot, O. Vavincq, G. Bouwnmans, A. Kudlinski, H. Rigneault
Journal of Biomedical Optics 19, 086021 (2014)
Rapidly tunable and compact coherent Raman scattering light source for molecular spectroscopy
S. Saint-Jalm, P. Berto, L. Jullien, E. R. Andresen, and H. Rigneault
Journal of Raman Spectroscopy 45, 515-520 (2014)
Background free stimulated Raman spectroscopy and microscopy
P. Berto, E. R. Andresen, H. Rigneault
Phys. Rev. Lett 112, 053905 (2014)
2013
Wide-field vibrational phase imaging in an extremely folded box-coherent anti-Stokes Raman scattering geometry
P. Berto, A. Jesacher, C. Roider, S. Monneret, H. Rigneault, and M. Ritsch-Marte
Optics Letters 38, 709-711 (2013)
2012
Fast and label free’, News and Views
H. Rigneault, E. Andresen
Nat. Phot 6, 802-803 (2012)
Wide-Field Vibrational Phase Imaging
P. Berto, D. Gachet, P. Bon, S. Monneret and H. Rigneault
Phys. Rev. Lett. 109, 093902 (2012)
Polarization resolved stimulated Raman scattering : probing depolarization ratios of liquids
F. Munhoz, S. Brustlein, R. Hostein, P. Berto, S. Brasselet and H. Rigneault
Journal of Raman Spectroscopy 43, 419–424 (2012)
2011
Detection of chemical interfaces in coherent anti-Stokes Raman scattering microscopy : D-CARS. II. Arbitrary interfaces
D. Gachet, H. Rigneault
J. Opt. Soc Am. A 28, pp. 2531-2539 (2011)
Detection of chemical interfaces in coherent anti-Stokes Raman scattering microscopy : Dk-CARS. I. Axial interfaces
D. Gachet, H. Rigneault
J. Opt. Soc Am. A 28, pp.2519-2530 (2011)
Transverse chemical interface detection with coherent anti-Stokes Raman scattering microscopy
S. Brustlein ; D. Gachet ; F. Billard ; H. Rigneault
Journal of Biomedical Optics 16, 086006 (2011)
Stimulated-Raman scattering microscopy by spectral focussing and fiber-generated soliton as Stokes pulse
E.R. Andresen, P. Berto, H. Rigneault
Opt Lett 36, 2387-2389 (2011)
Optical Parametric Oscillators based light source for Coherent Raman Scattering microscopy : a practical overview
S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet and H Rigneault
J. Biomed. Opt. 16, 021106 (2011)
Polarized multiplex CARS using a picosecond laser and a fiber supercontinuum
S. Michel, A. Courjaud, E. Mottay, J. Dudley, C. Finot, H. Rigneault
J. Biomed. Opt. 16, 021108 (2011)
2010
High Order Symmetry Structural Properties of Vibrational Resonances Using Multiple-Field Polarization Coherent Anti-Stokes Raman Spectroscopy Microscopy
F. Munhoz, H. Rigneault, S. Brasselet
Phys. Rev. Lett. 105, 123903 (2010)
Revisiting the Young’s Double Slit Experiment for Background-Free Nonlinear Raman Spectroscopy and Microscopy
D. Gachet, S. Brustlein, H. Rigneault
Phys. Rev. Lett. 104, pp.213905 (2010)
2009
Coherent anti-Stokes Raman scattering in a microcavity
David Gachet, Franck Billard, and Hervé Rigneault
Opt. Lett. 34, 1789-1791 (2009)
Coherent anti-Stokes Raman scattering in a Fabry-Perot cavity : A theoretical study
F. Billard, D. Gachet, and H. Rigneault
J. Opt. Soc. Am. B 26, 1295-1309 (2009)
Raman depolarization ratio of liquids probed by linear polarization Coherent Anti-Stokes Raman spectroscopy
F. Munhoz, S. Brustlein, D. Gachet, F. Billard, S. Brasselet and H. Rigneault
Journal of Raman Spectroscopy 40, 775-780 (2009)
2008
Background-free coherent anti-stokes Raman spectroscopy near transverse interfaces : a vectorial study
D. Gachet, F. Billard, H. Rigneault
J. Opt. Soc. Am. B 25, 1655-1666 (2008)
Focused field symmetries for background-free coherent anti-Stokes Raman spectroscopy
D. Gachet, F. Billard, and H. Rigneault
Phys. Rev. A 77, 061802(R) (2008)
2007
Coherent anti-Stokes Raman scattering (CARS) microscopy imaging at interfaces : evidence of interference effects
D. Gachet, F. Billard, N. Sandeau and H. Rigneault
Optics Express 15 (16), 10408-10420 (2007)
Coherent anti-Stokes Raman scattering microscopy (CARS) : Instrumentation and applications
N. Djaker, P.-F. Lenne, D. Marguet, A. Colonna, C. Hadjur, H. Rigneault
Nuclear Instruments and Methods in Physics Research A 571, 177–181 (2007)
2006
Microscopie Raman stimulée (CARS) : principes et applications
N. Djaker, D. Marguet, H. Rigneault
Médecine-Sciences 22 (10), pp853-858 (2006)
Influence of the Raman depolarisation ratio on far-field radiation patterns in coherent anti-Stokes Raman scattering (CARS) microscopy
D. Gachet, N. Sandeau, H. Rigneault
J. Europ. Opt. Soc. Rap. Com 1, 6013 (2006)
Refractive effects in coherent anti-Stokes Raman scattering microscopy
N. Djaker, D. Gachet, N. Sandeau, P.-F. Lenne, and H. Rigneault
Applied Optics 45 (27), 7005-7011 (2006)