| Contact |
| phone : +33 4 91 28 80 52 |
| fax : + 33 4 91 28 80 67 |
| e-mail : serge.monneret@fresnel.fr |
Serge
MONNERET, PhD
Microstereolithography
and related microfabrication processes
In the past (DCPR Nancy,
1997-2003), I studied
microfabrication processes based on three-dimensional
light-induced
polymerisation chemical reactions. Most of the results concerned a
microstereolithography apparatus for the manufacture of ceramic
microcomponents
with complex shapes. But image upconversion from the visible to the UV
domain
using nonlinear optics was also developed to allow dynamic
liquid-crystal-based
masks to be employed in a process working in the UV range. At
last, "nanofabrication" of phase plates with the use
of
evanescent fields has been demonstrated.
Combining
three-dimensional fluidic reservoirs and optical tweezers to control
beads/living cells contacts
Quantitative phase imaging of living cells
This is a new project with the french company PHASICS (Palaiseau). We would like to use their high resolution wave front sensor to make quantitative phase images of living cells. PHASICS SID4 product line uses a technology based on a modified Hartmann test to measure wavefront distortions. Using the multi-wave lateral shearing interferometry formalism to analyse the recorded Hartmanngrams leads to increased resolution (at least by a factor 4) compared to all other gradient recovery based wave front sensors (Hartmann test, Shack-Hartmann).Structured illumination on nanopatterned substrates
The aim of the present project
is to
develop a simple optical imaging system with sub-100 nm resolution
particularly
useful for the analysis of living cells. Some optical techniques
allowing
sub-diffraction imaging have been described. However, they always
involve sophisticated
equipments and are not readily available. Our optical system rests on
two main
ideas. The first one is to replace the glass slide of classical
microscopes by
a sub-wavelength grating substrate. This grating converts the incident
beam
into evanescent waves with high spatial frequencies that are able to
probe the
finest features of the sample. The second idea is to control the
amplitude and
phase of the plane waves forming the incident beam. Depending on the
illumination mode, one can shine the sample with a sub-wavelength light
grid or
scan it continuously with a ~100 nm light spot. This system should
allow
super-resolved wide-field imaging and, in combination with Fluorescence
Correlation Spectroscopy reading, should permit the study of dynamic
processes
at nanometre scales. Our first application will be to investigate
phenomena
occurring on the plasma membrane of live cells.
Selected recent publications
Highly flexible whole-field sectioning microscope with liquid-crystal light modulator
S. Monneret, M. Rauzi, P.-F. Lenne
J. Opt. A: Pure Appl. Opt 8, 6 pages (2006).
Practical lab tool for living cells based on microstereolithography and multiple dynamic holographic optical tweezers
S. Monneret, F. Belloni, D. Marguet
Proceedings of the SPIE, vol 6088 (invited paper) (2006).
Complex three-dimensional fluidic reservoirs to control beads/living cells contacts
S. Monneret, F. Belloni, O. Soppera
Microfluidics and Nanofluidics 3(6), 645-652 (2007).
Quadrant Kinoforms: an approach to multi-plane dynamic three-dimensional holographic trapping
F. Belloni,
Applied Optics 46 (21), 4587-4593 (2007).
Multiple holographic optical tweezers parallel calibration with optical potential well characterization
F. Belloni,
Optics Express 16 (12), 9011-9020 (2008).
Complete list of publications
S. Monneret, "La microstéréolithographie et ses applications", Mécanique & Industrie 6, 457-462 (2005).
S. Monneret, "Microfabrication directe de pièces céramiques tridimensionnelles de formes complexes", Techniques de l'Ingénieur (2004)
C. Provin, S. Monneret, H. Le Gall, S. Corbel, "Three-dimensional ceramic microcomponents made using microstereolithography", Advanced Materials 15 (12), 994-997 (2003).