Principal investigator : Julien Duboisset
Key words : Coherent anti-Stokes Raman scattering, spontaneous Raman, polarization, molecular order, vibrational modes.
We are particularly interested in investigate the molecular vibration for biological imaging and fundamental nonlinear spectroscopy. We develop method based on spontaneous Raman and coherent Raman scattering to directly image the symmetry of molecules in crystals and biological tissues, based on irreducible tensor formalism. (...)
Accueil > L’Institut > Équipes > MOSAIC > Thématiques
Thématiques
-
Non-linear optics for molecular spectroscopy
1er avril 2016 -
New fiber probes
1er avril 2016for biosensing and imaging
Principal investigator : Hervé Rigneault
keywords : nonlinear endoscope, lensless endoscope, wavefront shaping, micro-structured optical fibers, Raman probes
We are interested in extending molecular spectroscopy and nonlinear imaging through flexible fiber probes and endoscopes. Using specially design micro-structured fibers, we are investigating (1) the use of hollow core photonic crystal fibers for flexible nonlinear imaging endoscopes and Raman probes. (...) -
Quantitative phase microscopy for Biology
1er avril 2016using wavefront imaging
Principal investigators : Serge Monneret, Guillaume Baffou, Julien Savatier
keywords : lateral shearing interferometry, wavefront microscopy
Quantitative phase imaging (QPI) techniques are now conventionally used in microscopy for measuring specific properties of semi-transparent samples without any labelling. By placing a wavefront imaging system on the exit image plane of a microscope, we measure the complex field spatial distribution in this plane, and then (...) -
Non-linear optics for label-free microscopy
1er avril 2016Principal investigators : Hervé Rigneault, Sandro Heuke, Sophie Brasselet, Julien Duboisset
keywords : 2-photon fluorescence, second and third harmonic generation, coherent Raman scattering, pump-probe, nonlinear imaging, molecular spectroscopy
We are interested in how nonlinear optical contrast mechanisms can provide novel molecular information in microscopy and micro-spectroscopy. These modalities take advantage of label free capabilities, deep penetration into scattering samples, as (...) -
Wavefront shaping in scattering media
1er avril 2016for in-depth nonlinear imaging in tissues
Principal investigators : Sophie Brasselet
keywords:in-depth two-photon and coherent Raman scattering (CRS) imaging, wavefront shaping, adaptive optics
The potential of label-free imaging techniques based on nonlinear optics presents limits in turbid or heterogeneous media like biological tissues. Thanks to recent advances in wavefront control technologies (spatial light modulators based on liquid crystals, deformable mirrors), imaging in (...) -
Polarized microscopy
1er avril 2016from single molecules to tissues
Principal investigators : Sophie Brasselet
keywords : polarized fluorescence, polarized super-resolution imaging, polarized nonlinear microscopy
Polarization is an important property of light that is often ignored in light-matter interaction, in particular for bio-imaging applications. We develop methodologies for optical microscopy that exploit the properties of light polarization by a control of the incident or detected polarization states. This (...) -
Microspheres and single molecule detection
1er avril 2016Principal investigator : Jerome Wenger
Complex optical instrumentation, and/or expensive nanofabrication can hinder the progress of photonic technologies. Here we demonstrate that dielectric microspheres offer a relevant cost-effective alternative to more advanced lenses and objectives.
Beating the diffraction barrier with microspheres
Commercially available microspheres under focused Gaussian illumination can achieve three-axis optical confinement below the diffraction limit, with (...) -
Nanoaperture enhanced fluorescence
1er avril 2016Metal nanoapertures and zero-mode waveguides
Principal investigator : Jerome Wenger
Milling a nanometer-size aperture in a metallic film is an intuitive way to fabricate nanophotonic devices. Although the concept appears very simple, such apertures (also known as zero-mode waveguides) exhibit attractive properties for biophotonics : localization of excitation light strong isolation from emission produced by species located outside the aperture enhancement in the fluorescence signal (...) -
Plasmonic enhanced energy transfer
1er avril 2016Accelerating energy transfer between molecules with optical nanoantennas
Principal investigator : Jerome Wenger
Energy transfer between molecules is promoted when they are set in an environment that confines light. The energy transfer between molecules is an essential phenomenon for photosynthesis, photovoltaics and biotechnology. Now, thanks to the work of the Institut Fresnel Institute, energy transfer between molecules can be controlled and enhanced with optical structures etched at (...) -
Nanoantenna enhanced fluorescence
1er avril 2016Antennas in nanobiophotonics
Principal investigator : Jerome Wenger
Nanophotonics can improve single molecule optical detection beyond the diffraction limit. The main goal is to tailor the electromagnetic environment by the use of an optical antenna to concentrate the light on a tiny spot and simultaneously enhance the molecular emission. Single molecule spectroscopy techniques, FRET and FCS can greatly benefit from photonic nanoantennas to enter a new dimension of higher (...)
- 1
- 2